Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Cancer therapy via neoepitope-specific monoclonal antibody cocktails

doi: 10.1007/s00262-025-04075-3

Figure Lengend Snippet: Overview of the Fab phage display library screening pipeline. A Graphical schematic showing the nonimmune, human, Fab-expressing phage display library format and reformatting of selected clones as antibodies. B List of four neoepitope 11-mer peptides screened against the phage display library and resulting clones (P1–P4). C Process flowchart showing the sequential steps in the isolation of Fab fragments specific for each peptide from the phage display library. BSA, bovine serum albumin; KLH, keyhole limpet hemocyanin

Article Snippet: Three rounds of panning were conducted with either peptide alone or with peptide conjugations to BSA, BSA-biotin, or KLH at Sanyou Biopharmaceuticals.

Techniques: Library Screening, Expressing, Clone Assay, Isolation

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Cancer therapy via neoepitope-specific monoclonal antibody cocktails

doi: 10.1007/s00262-025-04075-3

Figure Lengend Snippet: Neoepitope-targeted phage-derived scFv-Fc antibodies bind to B16F10-mutated peptides and cells and elicit effector functions. A , B Binding of phage-derived (P) antibodies (P1–P4) to wild-type (hollow) and mutant (filled) peptides by multiplex assay ( A ) and ELISA ( B ). C Immunohistochemistry staining of B16F10 cells. D Flow cytometry binding of yeast-derived antibodies for target mouse (red) and human (blue) cell lines. E Antibody-dependent FcγRIIIa signaling measured using Jurkat-Lucia NFAT-CD16 reporter cells at a 4:1 E:T ratio. F Antibody-dependent phagocytosis of B16F10 cells with THP-1 monocytes at a 1:1 E:T ratio. G Antibody-induced FcγRIIIa signaling measured using Jurkat-Lucia NFAT-CD16 reporter cells for target mouse (red) and human (blue) cell lines at a 4:1 E:T ratio. A one-way ANOVA ( F 6,14 = 16.78, p < 0.0001) followed by Dunnett's post hoc test was used to compare groups against the isotype control (ns p ≥ 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Error bars represent SD of 3 replicates. Phage-derived antibodies with unmodified Fc domains are indicated as P and Fc-enhanced Fc domains as P E . MFI, median fluorescence intensity; EC 50 , half-maximal effective concentration, BSA, bovine serum albumin; KLH, keyhole limpet hemocyanin; RLU, relative light units; Rb pAb, positive control rabbit polyclonal antibody cocktail

Article Snippet: Three rounds of panning were conducted with either peptide alone or with peptide conjugations to BSA, BSA-biotin, or KLH at Sanyou Biopharmaceuticals.

Techniques: Derivative Assay, Binding Assay, Mutagenesis, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Staining, Flow Cytometry, Control, Fluorescence, Concentration Assay, Positive Control